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recombinant ifn α  (Miltenyi Biotec)


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    Miltenyi Biotec recombinant ifn α
    Recombinant Ifn α, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+ifn+%CE%B12b/Human+IFN-%CE%B12b%2C+research+grade/pm42328836-83-4-6
    Average 94 stars, based on 5 article reviews
    recombinant ifn α - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Staining:

    Article Title: Lipid deprivation amplifies type I IFN responses in monocytes through prenylation: insights from familial combined hypolipidemia type 2
    Article Snippet: Intracellular staining was performed for 30 min at room temperature after fixation and permeabilization with the FOXP3/Transcription Factor Staining Buffer Set according to the manufacturer’s instructions (Thermo Fisher Scientific) using ISG15 PE (R&D Systems cat. IC8044P). .. In some experiments, cells were stimulated 18 h with human IFN-α2b (Miltenyi Biotec 130-093-875) 40.000 U/mL before staining. .. For the analysis of STAT1 phosphorylation, cells were stimulated with IFN-α2b 40.000 U/ml (Miltenyi Biotec) for 15 min at 37 °C, then fixed and permeabilized using Cytofix Fixation Buffer (BD Biosciences, pre-warmed to 37 °C) and Perm Buffer III (BD Biosciences, pre-cooled to -20 °C), according to the instructions of the manufacturer.

    Article Title: Lipid deprivation amplifies type I IFN responses in monocytes through prenylation: insights from familial combined hypolipidemia type 2.
    Article Snippet: 26 Intracellular staining was performed for 30 min at room temperature after 27 fixation and permeabilization with the FOXP3/Transcription Factor 28 Staining Buffer Set according to the manufacturer’s instructions (Thermo 29 Fisher Scientific) using ISG15 PE (R&D Systems cat. IC8044P), or 30 Phospho-S6 Ribosomal Protein (Ser235/236) APC (Cell Signaling 31 Technology cat. 14733S). .. In some experiments, cells were stimulated 18 32 hours with human IFN-α2b (Miltenyi Biotec 130-093-875) 40.000 U/mL 33 before staining. .. 34 For the analysis of STAT1 phosphorylation, cells were stimulated with IFN35 α2b 40.000 U/ml (Miltenyi Biotec) for 15 min at 37°C, then fixed and 36 permeabilized using Cytofix Fixation Buffer (BD Biosciences, pre-warmed 37 to 37°C) and Perm Buffer III (BD Biosciences, pre-cooled to -20°C), 38 according to the instructions of the manufacturer.



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    Image Search Results


    Correlation between AAb-IFN-α levels and GADA. Correlation between levels of AAb-IFN-α isoform 2b and GAD65 antibodies levels in individuals with LADA who tested positive for AAb-IFN-α2. Spearman's test was fitted to assess the monotonic covariation of measurements. Abbreviations: AAb-IFN-α, autoantibodies against interferon-α; GADA, glutamic acid decarboxylase antibodies; LADA, latent autoimmune diabetes in adults.

    Journal: The Journal of Clinical Endocrinology and Metabolism

    Article Title: IFN-α Neutralizing Antibodies Distinguish LADA From Early-onset Type 1 Diabetes

    doi: 10.1210/clinem/dgaf001

    Figure Lengend Snippet: Correlation between AAb-IFN-α levels and GADA. Correlation between levels of AAb-IFN-α isoform 2b and GAD65 antibodies levels in individuals with LADA who tested positive for AAb-IFN-α2. Spearman's test was fitted to assess the monotonic covariation of measurements. Abbreviations: AAb-IFN-α, autoantibodies against interferon-α; GADA, glutamic acid decarboxylase antibodies; LADA, latent autoimmune diabetes in adults.

    Article Snippet: The antibodies directed against the following 3 isoforms of IFN-α were evaluated in sera of study participants: IFN-α1 (InvivoGen; catalog no. rcyc-hifna1), IFN-α2b (InvivoGen; catalog no. rcyc-hifna2b), IFN-α14 (PBL Assay Science, Piscataway, NJ, USA; catalog no. 11145).

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